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課程名稱 |
膜蛋白動力學
Membrane Protein Kinetics |
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開課學期 |
105-1 |
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授課對象 |
生物資源暨農學院 獸醫學研究所 |
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授課教師 |
蔡沛學 |
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課號 |
VM7098 |
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課程識別碼 |
629EM2650 |
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班次 |
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學分 |
2 |
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全/半年 |
半年 |
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必/選修 |
選修 |
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上課時間 |
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上課地點 |
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備註 |
本課程以英語授課。英文授課。
限碩士班以上
總人數上限：8人
外系人數限制：2人 |
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Ceiba 課程網頁 |
http://ceiba.ntu.edu.tw/1051VM7098_ |
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課程簡介影片 |
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核心能力關聯 |
本課程尚未建立核心能力關連 |
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課程大綱
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為確保您我的權利,請尊重智慧財產權及不得非法影印
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課程概述 |
1. Course Introduction
2. The Structure and the Properties of Biological Membrane
3. Membrane proteins (I)
4. Membrane proteins (II)
5. Moving Proteins into Membranes
6. Endocytosis of Membrane Proteins
7. Mechanisms for Protein Translocation (I)
8. Mechanisms for Protein Translocation (II)
9. Mid-term week (suspend)
10. Guest lecture
11. Isolation and Visualization of Membrane Proteins (I)
12. Isolation and Visualization of Membrane Proteins (II)
13. Recent Breakthrough Technologies for Studying Membrane Protein (including literature discussion)
14. Experimental Designs for Membrane Proteins
15. Lab Practice I (Isolation of Membrane Proteins)
16. Lab Practice II (Visualization of Membrane Proteins)
17. Problems Shooting/Discussion (whole class)
18. Final Report
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課程目標 |
We aim to allow our students to have the ability to properly design, correctly precede specific experiments when they deal with membrane proteins and related subjects. In class, we combine in class lecture, lab practice (allow students to work on their own specific experiments that directly linked to their master or PhD study) as well as interactive discussions in order to have direct and fast feedback on questions and difficulties they encounter.
這門課希望透過課堂lecture結合實習（實驗）與互動性的討論方式，幫助碩士或博士班學生針對細胞膜蛋白相關實驗進行設計、執行（進行）並解決實驗上所可能遇到之困難。
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課程要求 |
Active participate class discussion
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預期每週課後學習時數 |
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Office Hours |
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指定閱讀 |
1.Molecular Biology of the Cell (B. Albert et. al, 5th edition), Garland Science
2.Molecular Cell Biology (H. Lodish et al, 7th edition), Freeman
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參考書目 |
1.Molecular Biology of the Cell (B. Albert et. al, 5th edition), Garland Science
2.Molecular Cell Biology (H. Lodish et al, 7th edition), Freeman
3.The Molecular Probes Handbook (I. Johnson, M.T.Z Spence et. al 11th edition, Invitrogen)
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評量方式
(僅供參考) |
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No. |
項目 |
百分比 |
說明 |
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1. |
Midterm exam |
30% |
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2. |
Final Report |
40% |
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3. |
Class participation |
30% |
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週次 |
日期 |
單元主題 |
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第1週 |
9/16 |
Introduction (postponed due to National Holiday) |
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第2週 |
9/23 |
Introduction/ Visualizing what you can’t see from your naked eyes |
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第3週 |
9/30 |
Personal discussion 1 |
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第4週 |
10/7 |
Visualizing what you can’t see from your naked eyes (II) |
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第5週 |
10/14 |
The structure and the properties of biological membrane |
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第6週 |
10/21 |
Personal discussion 2 |
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第7週 |
10/28 |
Personal discussion 3 |
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第8週 |
11/4 |
Moving proteins into membranes |
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第9週 |
11/11 |
Enocytosis of surface molecules |
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第10週 |
11/18 |
Midterm
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第11週 |
11/25 |
Personal discussion 4 |
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第12週 |
12/02 |
Potein translocation: the cargo, the truck and the highway
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第13週 |
12/09 |
Personal discussion 5 |
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第14週 |
12/16 |
Recent breakthrough technologies for studying membrane proteins |
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第15週 |
12/23 |
Personal discussion 6 |
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第16週 |
12/30 |
Final evaluation |